Fig. 4. Etanercept prevents neutrophil extracellular trap (NET) formation in injured serosa. Mice were administered phosphate-buffered saline (PBS) (A) or etanercept (100 mg/mouse) (B). The cecal lesions were sampled at 24 h after cecum cauterization for HE staining or immunofluorescence for Ly6G (red), citrullinated histone 3 (CitH3) (green), and DAPI (blue). Photographs with low (upper panels) and high (lower panels) magnifications are shown. Three mice were used for each experimental group. We performed the experiments three times with similar results.